NCoR1/HDAC3 corepressor complex could block myeloid differentiation in MLL-AF9–induced AML. (A) FACS analysis of CD11b⁺Gr-1^low, CD11b⁺Gr-1^intermediate, and CD11b⁺Gr-1^high populations in GFP⁺ leukemia cells from PB and BM of secondary recipients (n = 5 per group) 20 days after transplantation. Representative FACS profiles are shown (left panel). (B) Real-time qPCR analysis of myeloid differentiation factors in BM leukemia cells of secondary recipients (n = 5 per group) 20 days after transplantation. mRNA levels were normalized to Gapdh expression. ChIP analysis of the Cebpe (left panels) and Mpo (right panels) promoters using anti-NCoR1 (C), anti-HDAC3 (D), and the indicated antibodies (E) in BM leukemia cells of secondary recipients 20 days after transplantation. IgG served as a negative control. Data are mean ± standard error of the mean from 3 independent experiments. *P < .05, **P < .01, ***P < .001.