PDPN⁺and PDPN⁻orthotopic gliomas resemble human GBM features. OLIG2, Ki67, and PDPN expression (brown) in brain sections from DKO-NSCs (A) and TKO^{Pdpn neg}-NSCs (B) orthotopic gliomas detected by immunohistochemistry. The sections were counterstained with hematoxylin. (C) Immunofluorescence staining for Ki67 and PDPN of tumor cells isolated from mice transplanted orthotopically with either DKO-NSCs or TKO^{Pdpn neg}-NSCs. Cellular nuclei are stained with DAPI and pseudocolored in blue. Scale bars, 25 µm.