Expression of CD4Treg functional markers during IL-2 therapy. (A) FOXP3 expression in naive and memory CD4Treg subsets. (B) Increase in BCL-2 expression from week 1 (W1) to week 12 (W12) in clinical responders and nonresponders for naive and memory CD4Treg subsets. (C) Increase in FOXP3 expression from baseline (W0) to week 1 (W1) in clinical responders and nonresponders for naive and memory CD4Treg subsets. (D) BCL-2 expression in naive and memory CD4Treg subsets. (A-B) Median values and the interquartile range (whisker bars) are shown for each parameter. Time points represent weeks (W) and months (M) after IL-2 initiation. Number of patients evaluated at each time point is indicated at the bottom of the image. (C-D) Dots depict individual patients. Lines connect the median values at each time point. The Wilcoxon rank-sum test was used to compare responders vs nonresponders. n.s., not significant.