Clone sharing between different T-cell populations. (A) TCR overlap (Jaccard overlap index) between CD4Treg and CD4Tcon repertoires at baseline (W0), week 12 (W12), and 1 year (1Y). Overlap in HDs (gray) is shown for comparison. (B) Percentage of the total unique clones at week 12 (W12) that were detected within different baseline (W0) populations. Each column shows the percentage of week 12 clones within the various cell types that are exclusively shared with a particular baseline population. For example, the first column on the left shows the percentage of week 12 CD4Tregs clones shared exclusively with the baseline CD4Tcon population. There is significantly higher clone sharing between CD4Tregs and CD4Tcons than between CD4Tregs and CD8 T cells. In both panels, each dot represents a patient (n = 12) or an HD (n = 8). Box plots depict the 75th percentile, median, and 25th percentile values; whiskers represent maximum and minimum values, except for outliers. Two-sided Wilcoxon signed-rank test was used to assess significance between the different combinations.