Identification of a novel HBO1-fusion in a patient with CMML. (A) BM smear from the patient with CMML. Scale bar, 20 µm (original magnification ×1000; Wright-Giemsa stain). (B) G-banding karyotyping analysis of BM cells from the patient with CMML. Red triangles indicate the location of chromosomal translocation. (C) FISH analysis of BM cells from the patient with CMML. (D) Schematic of the CMML patient-derived NUP98-HBO1 fusion. (E) Schematic of the retrovirus vectors. (F) Expression of the NUP98-HBO1 fusion product in 293T cells. (G) Localization of the NUP98-HBO1 fusion protein. HeLa cells were transfected with the indicated vectors 24 hours before immunofluorescence staining. Scale bar, 20 µm (original magnification ×1000; immunofluorescence stain).