Combined FLT3 and JAK inhibition decreases leukemic burden in vivo. (A) Schematic of in vivo experimental design. Gilteritinib was administered by oral gavage at 60 mg/kg thrice weekly. Ruxolitinib was administered continuously by using a chow formulation (2000 mg/kg of chow). Luminescent imaging of NSG mice (B) or NSGS mice (D) at baseline and after 10 days of indicated treatment. A different color scale is used for the right panel in D to emphasize differences. Images using a uniform scale are presented in supplemental Figure 3. (C,E) Quantification of total flux for panels B and D, respectively. Dashed line represents average pretreatment baseline luminescence. (F) Percent change in body weight of NSGS mice after 10 days of indicated treatment. Error bars represent SEM. *P < .05; **P < .01; ***P < .001.