DGKζ deficiency improves platelet adhesion to collagen under conditions of flow. Whole blood collected from WT and DGKζ-KO mice was labeled with mepacrine and flowed over collagen-coated microchannels. (A) Representative images of platelet coverage under conditions of arterial shear (1500 s⁻¹). Images were taken under ×20 objective. (B) Surface area coverage at the indicated time points was quantified and reported as the mean ± SEM (n = 6). (C) Microchannels were washed after whole blood perfusion for 300 seconds and fixed, permeabilized, and stained with phalloidin-TRITC; Z stack images were then taken. (i) Representative Z stack images. Bars represent 100 μm. The volume (ii) and height (iii) of each platelet thrombus at 300 seconds were quantified and reported as the mean ± SEM (n = 5; 76-116 thrombi per sample). Statistical analysis was performed by the unpaired Student t test. *P < .05, **P < .01, ***P < .001 of DGKζ-KO as compared with WT.