Increased expression of GPVI in DGKζ-KO platelets. (A) Platelet surface receptor expression of GPVI (i-ii), integrin α2 (iii), integrin αIIb (iv), PECAM-1 (v), GPIbα (vi), and CLEC-2 (vii) was measured in diluted whole blood samples from WT and DGKζ-KO mice by flow cytometry. Mean fluorescence intensity (MFI) is reported as the mean ± SEM (n = 10). (B) Whole lysates of washed platelets from WT and DGKζ-KO mice were analyzed by western blotting for GPVI, GPIIIa, FcRγ chain, and actin. The blots are representative of 4 independent experiments. Quantification of band densities (right) is reported as the mean ± SEM (n = 4). Statistical analysis was performed using the unpaired Student t test. *P < .05, ****P < .0001 of DGKζ-KO as compared with WT platelets. FITC, fluorescein isothiocyanate; IgG, immunoglobulin G.