Identification of FVIII peptides on MoDCs obtained from HA patients and healthy blood donors using the MAPPs assay. (A) HLAcII-DRB1 alleles of subjects used in this study. The blue shading shows whether a particular allele was found in HA patients or healthy donors. Frequencies of each allele in various demographic groups are listed and identified on a scale from white (0) to red (15%). (B-C) Peptides identified in the MAPPs assay on DRB1-based DR molecules when MoDCs were incubated with FL-rFVIII plus pdVWF (B) or pdFVIII plus pdVWF (C). The peptides identified in cells from HA patients are shown in red; peptides identified in healthy donors are shown in blue. (D) Number of FVIII peptides identified on DR, DP, and DQ molecules in the MAPPs assay when APCs were incubated with FL-rFVIII plus pdVWF.