Comparison of FVIII peptides identified in a MAPPs assay following incubation of MoDCs with FL-rFVIII plus pdVWF or pdFVIII plus pdVWF. (A) For each donor and HA patient, number of peptides found in the MAPPs assay for FL-rFVIII plus pdVWF (red) and pdFVIII plus pdVWF (blue) are shown. In addition, the MHC-II–DRB1 alleles for each donor are identified as well as their respective frequencies in African, Asian, and white populations. (B) Location and frequencies of peptides for a single donor (D1179) identified in the MAPPs assay following incubation of APCs with FL-rFVIII plus pdVWF (blue) or pdFVIII plus pdVWF (red). (C) Pooled data for all subjects showing location and frequencies of peptides identified following incubation with FL-rFVIII plus pdVWF where no peptides were found after incubation with pdFVIII plus pdVWF.