HLAcII-DRB1 coverage in this study and comparison of FVIII peptides identified in this study and those identified in previous studies. (A) The bar labeled “World” represents a set of 38 MHC-II–DRB1 alleles and their respective frequencies in the world population; together, these alleles represent 95.42% of alleles found in this population. The bar labeled “Study” represents the 16 alleles found in the subjects of the MAPPS assays. This subset represents 59.89% of alleles found in the world population. The relative frequencies of the MHC-II–DRB1 alleles in the study population and the world population are comparable. (B) Previous studies have identified FVIII peptides in MAPPs assays using a BDD-rFVIII, whose primary amino acid sequence corresponds exactly to that of the BDD-rFVIII used in this study; the peptides identified in studies by Peyron et al²⁶  (green), van Haren et al²⁵  (blue), and in the current study are shown for comparison.