Single-cell lipid analysis of DP and DN thrombocytes by MALDI mass spectrometry. (A-B) Section of lipid profile mass spectrum peaks and their intensities obtained from high-intensity GFP⁺ thrombocytes (DN thrombocytes) and RFP⁺ low-intensity GFP⁺ thrombocytes (DP thrombocytes), respectively, by MALDI-LTQ-Orbitrap [M⁺Na]⁺ or [M⁺H]⁺. Labels on top of the peaks indicate m/z values. (C) Intensity of mass spectrum peaks representing lipids that were >1%, from DP and DN thrombocytes shown in Table 1. The numbers adjacent to the listed lipids are m/z values. The peaks from DN and DP thrombocytes for glycerophospholipids (PE) with an m/z value of 859.61 and glycerophospholipids (PC) with an m/z value of 860.62 showed significant differences. The respective P values are P < .001 and P < .007 for 3 replicates. The comparison of peak intensities for the sphingolipid-glycerophospholipid with an m/z value of 867.67 did not show any significant difference (ns, not significant; P = .47). Error bars represent standard error of the mean.