Alterations in the CXCL12/CXCR4 axis in ITP patients. (A) BM CXCL12 concentration analyzed by ELISA in the ITP group (n = 30) and control group (n = 28). (B) PB CXCL12 concentration analyzed by ELISA in the ITP group (n = 30) and control group (n = 28). (C) BM TPO concentration analyzed by ELISA in the ITP group (n = 30) and control group (n = 28). (D) Percentage of CXCR4⁺ CD41⁺ MKs analyzed by FACS in the ITP group (n = 30) and control group (n = 28). (E) CXCR4 mRNA in BM MKs in the ITP group (n = 30) and control group (n = 28) analyzed by PCR. Data are pooled from 10 independent experiments with 4 to 8 samples per experiment. Data are shown as mean ± SD. Each point represents the mean adjusted value of 3 replicates for each individual patient. P values were calculated using the Mann-Whitney U test. *P < .001.