Reduced sympathetic innervation of BM nestin⁺MSCs in ITP patients. (A) Representative pictures of BM biopsy tissue from the control group immunohistochemically stained with an anti-NE antibody and hematoxylin and eosin. Scale bar, 100 μm. (B) Representative pictures of BM biopsy tissue from the ITP group immunohistochemically stained with an anti-NE antibody and hematoxylin and eosin. Scale bar, 100 μm. (C) Integral optical densities (IODs) of BM NE staining in the ITP group (n = 30) and control group (n = 28). (D) Percentage of β3-AR⁺ nestin⁺ MSCs in nestin⁺ MSCs analyzed by FACS in the ITP group (n = 30) and control group (n = 28). (E) β3-AR mRNA level in nestin⁺ MSCs in the ITP group (n = 30) and control group (n = 28). Data are pooled from 10 independent experiments with 4 to 8 samples per experiment (C-E). Data are shown as mean ± SD. Each point represents the mean adjusted value of 3 replicates for each individual patient. P values were calculated using the Mann-Whitney U test. *P < .001.