Relative ROS production of WBCs is reduced in Fibγ^390-396ASS mice compared with the Fib^WTSS mice. Blood samples from the experimental mice were collected 9 months after BMT and stained with RBCs, WBCs, and platelet-specific antibodies and ROS detection reagent (CM-H2-DCFDA). (A) Relative RBC ROS. (B) Relative WBC ROS. (C) Relative platelets ROS. Fib^WT B6 (n = 12-14), Fibγ^390-396A B6 (n = 11-14), Fib^WT SS (n = 12-14), and Fibγ^390-396A SS (n = 12-14). Similar number of fully chimeric male and female mice were used in the experimental groups. Each symbol in the graph represents an individual mouse. The bars in the graph indicate the mean ± SEM. Statistical significance was determined using 1-way ANOVA followed by the Tukey's multiple comparison test; statistical significance is indicated as **P ≤ .01, ****P ≤ .0001. (A) For RBC ROS, the Dunn's test was used; ***P ≤ .001. ns, not significant.