Memory phenotypes of NY-ESO-1 SPEAR T cells. (A) Distribution of memory subsets with described phenotypic markers of CM, EM, EM RA⁺ (EMRA), stem cell memory SCM, and naïve within CD8⁺ pentamer⁺ NY-ESO-1 SPEAR T cells in BM and PBMC postinfusion were performed in patients in whom parallel collection of BM and PBMC was carried out and in whom sufficient cells were available for analysis (n = 8). Pie chart colors represent the proportions of NY‐ESO‐1 SPEAR T‐cells with described phenotypic markers of CM (red), EM (blue), EMRA (green), SCM (orange), and naïve cells (purple) within CD8⁺ SPEAR T‐cells. (B) Percentage of NY-ESO-1 SPEAR T cells with described phenotypic markers of CM, EM, EMRA, and SCM within CD8⁺ pentamer⁺ NY-ESO-1 SPEAR T cells in the manufactured product and posttreatment PBMC at various times from all 25 patients. The dominant populations of persisting cells were EMRA (green) and SCM (orange). Box plot shows the mean and upper and lower quartiles.