Figure 2.
Figure 2. Generation and characterization of a Stat3β transgenic mouse model. (A) A schematic overview of the Stat3β transgene BAC construct. (B) Total cell lysates from spleen and liver were subjected to western blot analysis with the indicated antibodies (STAT3, FLAG, and HSP90). Liver sections stained with an antibody against FLAG show the expression of ectopic STAT3β. (C) A representative flow cytometry analysis of YFP+ BM cells (BMCs) and hematopoietic cell populations (progenitor: Lin−Sca-1+c-Kit−; LSK: Lin−Sca-1+c-Kit+) at day 20 postinduction. Data (wt vs Stat3βTG) were not statistically compared.

Generation and characterization of a Stat3β transgenic mouse model. (A) A schematic overview of the Stat3β transgene BAC construct. (B) Total cell lysates from spleen and liver were subjected to western blot analysis with the indicated antibodies (STAT3, FLAG, and HSP90). Liver sections stained with an antibody against FLAG show the expression of ectopic STAT3β. (C) A representative flow cytometry analysis of YFP+ BM cells (BMCs) and hematopoietic cell populations (progenitor: Lin−Sca-1+c-Kit−; LSK: Lin−Sca-1+c-Kit+) at day 20 postinduction. Data (wt vs Stat3βTG) were not statistically compared.

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