Overview of FOXO1 mutations in pediatric sporadic and endemic BL patients. The distribution of mutations in patient samples identified in the evolutionarily conserved cluster region (M1, R19-L27), including the AKT motif (R19-T24), are shown for the 23 of 78 FOXO1 mutated sporadic BL (sBL; red) (A) and the 48 of 89 FOXO1-mutated endemic BL (eBL; green) (B) samples, taken at initial diagnosis. Fourteen patients had multiple FOXO1 mutations: in 4 cases, 2 mutations were both located within the cluster region (as indicated), 3 had an S22 mutation with a second mutation located outside the cluster region and the remaining 7 cases had 2 or more mutations located outside the cluster region only. (C) Mutation lolliplot showing the positions of all mutations detected in the FOXO1 protein relative to the phosphorylation sites T24, S256, and S319 (yellow) and protein domains. Mutations in the sBL cohort are shown at top in red and in the eBL cohort at bottom in green.