IRF4^hiCD8⁺T cells highly express CD25. Healthy donor PBMCs were stimulated with CEF peptides for 18 hours. (A) Mean fluorescence intensity of CD25, CD69, and CD137 in IRF4^neg, IRF4^lo, IRF4^int, and IRF4^hi CD137⁺ populations. (B) A representative viSNE analysis of CEF-specific CD8⁺ T cells (gated on CD8⁺CD69⁺ cells). Each point represents a single cell, and different colors represent the intensity of the expression of the molecule. CEF-specific CD8⁺ T cells (IRF4⁺CD137⁺) are indicated by the oval.