CD8 cell dynamics. Irradiated 129 mice were reconstituted with B6 CD11c-YFP BM, B6 RFP⁺ CD8 cells, and unlabeled B6 CD4 cells. Ears were imaged ∼28 days after transplantation. (A) Image from a representative video (supplemental Video 2). Mean speeds and displacements for tracked cells are shown in panel B. Each symbol is a unique tracked CD8 cell. (C) Representative tracking from a single video with the starting point of each CD8 cell at the origin. Color denotes time. (D) Mean speed, distance to the YFP surface, and displacement were calculated for each cell in 3 videos (color coded). (E) Average CD8 cell behaviors from 3 videos, represented in a 100-circle plot.