mito-Dendra2^hiHSCs have higher mitochondrial mass than mito-Dendra2^loHSCs. (A) Representative flow cytometric graph for gating of mito-Dendra2^lo (bottom 10%) and mito-Dendra2^hi (top 10%) fractions within BM HSCs (SLAM LSK cells) obtained from mito-Dendra2 mice. (B) Representative superresolution images of fluorescent microspheres (diameter, 0.18 μm) before (left panel) and after (right panel) PSF correction. (C) Representative isosurface rendering of PSF-corrected superresolution images of mito-Dendra2^lo (left panel) and mito-Dendra2^hi (right panel) HSCs. Scale bar, 2 μm. (D) Mitochondrial mass in mito-Dendra2^lo and mito-Dendra2^hi HSCs determined by superresolution images with PSF correction (mean ± standard error of the mean [SEM]; n > 60 each). (E) Schematic flow showing the absolute quantification of mitochondrial DNA copy number (mtDNA/nDNA) using ddPCR. (F) Mitochondrial DNA copy number in mito-Dendra2^lo and mito-Dendra2^hi HSCs determined by ddPCR (mean ± SEM; n = 7 each). (G) Representative flow cytometric graph of CellROX fluorescence intensity in mito-Dendra2^lo and mito-Dendra2^hi HSCs. (H) Cellular ROS levels in mito-Dendra2^lo and mito-Dendra2^hi HSCs (mean ± standard deviation [SD]; n = 3 each). *P < .05, **P < .01, ****P < .0001, Student t test.