HJB-RBCs and ISCs in children with SCA. (A) %HJB-RBCs determined by IFC in 45 children at 3 to 6 and 18 months. ****P < .0001, Wilcoxon paired test. (B) Distribution of %HJB-RBCs with respect to splenic uptake as measured by ^99mTc heated RBCs spleen scintigraphy. Splenic uptake of 100% to 150% (n = 15), 75% to 100% (n = 4), 50% to 75% (n = 1), 25% to 50% (n = 2), and 0% to 25% (n = 5). (C) %ISCs determined by IFC in 45 children at 3 to 6 and 18 months. ****P < .0001, Wilcoxon paired test. (D) Spearman correlation between %ISCs and %HJB-RBCs at 3 to 6 (red dots) and 18 months (blue dots). n = 99; R² = 0.69; ***P < .001.