Overexpression of SPOP mutant increases γ-globin. (A) HbF flow cytometry of differentiated HUDEP-2 cells expressing empty vector (left) or the SPOPY87N mutant. (B) Western blot analysis with indicated antibodies for differentiated HUDEP-2 cells expressing empty vector or SPOP mutant. (C) γ-Globin mRNA was measured by RT-qPCR; data are plotted as percentage of γ-globin over γ-globin + β-globin. Data are mean ± SD of 2 biological replicates. ***P < .01, unpaired Student t tests.