SPOP depletion strongly enhances the effect on γ-globin induction by pomalidomide treatment. (A) HbF flow cytometry of indicated HUDEP-2 sgRNAs cell pools treated with or without pomalidomide (Pom) at day 6 of differentiation. (B) γ-Globin mRNA was measured by RT-qPCR; data are plotted as percentage of γ-globin over γ-globin + β-globin. Data are mean ± SD of 2 independent experiments. (C) BCL11A mRNA levels measured by RT-qPCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used for normalization. Data are mean ± SD of 2 biological replicates. **P < .05; ***P < .01, unpaired Student t tests.