Defective hemostasis and thrombus growth in DKO mice. (A) Bleeding time measured as the time to the first cessation of bleeding. For DKO mice, bleeding was manually stopped at 1800 seconds; n = 10-12 mice, scatter plot with mean ± SEM, each dot corresponds to an individual mouse, ***P < .0001 with Kruskal-Wallis test and Dunn’s multiple comparison test. (B) TF-induced thromboembolism experiments. (Left panel) Percentage survival following tissue factor injection, n = 10 mice; statistics using a log-rank (Mantel-Cox) test. (Right panel) Individual and mean percentage of platelet consumption, n = 8-9 mice; **P < .01 using Student t test. (C) Laser-induced mesenteric arteriole injury. (Left panel) Curves representing the mean ± SEM thrombus area at each time point of 10 to 13 vessels observed from 7 to 9 mice. (Right panel) Bar graph of the area under the curve (AUC) of the individual curves corresponding to the graph shown in left. **P < .001, Kruskal-Wallis test. (D) FeCl₃-induced carotid artery injury. FeCl₃ was applied to the lateral side of the carotid for 150 seconds (arrow), after which thrombus growth was visualized from the top. (Upper left panel) Curves representing the mean ± SEM thrombus area at each time point, n = 10 mice. (Upper right panel) Representative top view showing the fluorescent platelet accumulation at the peak thrombus formation in WT mice (748 seconds) (upper image) and the absence of platelet accumulation in DKO mice (lower image). (Lower panel) Scatter bar graph of the AUC; **P < .001, ***P < .0001 using Kruskal-Wallis test and Dunn’s posttests.