Transplantation of bone marrow cells from WT mice rescues the impaired platelet functions of Nix^−/−mice Bone marrow was isolated from Nix^−/− mice and WT mice. The prepared bone marrow samples were transplanted into recipient mice as indicated in the figures. (A) Statistical analysis of tail-bleeding times of the transplanted Nix^−/− mice and WT mice. (B) Statistical analysis of occlusion time in the FeCl₃-induced thrombus model in transplanted Nix^−/− mice and WT mice. (C-D) Platelet aggregation induced by thrombin was monitored (C) and the aggregation amplitudes were analyzed (D). Baseline of platelet aggregation trace was indicated with arrowhead. (E-F) P-selectin surface expression and α_IIbβ₃ activation were analyzed by flow cytometry in platelets stimulated with 0.05 U/mL α-thrombin from the transplanted mice using antibodies against P-selectin (E) and the active α_IIbβ₃ (F), respectively. Statistical analysis of the data are shown. (G) OCR of the platelets from transplanted Nix^−/− and WT mice. Mitochondrial OCR was measured by Seahorse analysis. (H) ATP production capacity of platelets from transplanted Nix^−/− and WT mice. (I-J) Induction of mitophagy in FCCP-treated platelets from transplanted mice was detected by western blotting using the indicated antibodies (I). (J) The grayscale values of all the bands were measured with ImageJ software and the statistical data are illustrated. ANOVA was used. *P < .05; **P < .01; n = 6 for each group if it is not stated in the figures.