Statistical analysis of metabolic clustering and carry forward with congenic and Steap3 transgenic mice. (A) Principle component analysis of 3 separate experiments for each of the indicated mouse strains. (B) Examples of analytes that carry forward (eg, 9-HODE) and those that do not (eg, hypoxanthine, adenosine triphosphate (ATP), and glutathione (GSH). (C) Volcano plots showing the metabolomics of stored RBCs from experimental strains compared with B6, depicting the general extent of clustering of statistically significant analytes that carry forward into congenic and Steap3 transgenic strains.