Posttransplant numbers of blood lymphocytes and DCs are suppressed by the occurrence of grade 2-4 aGVHD in BM recipients, whereas functional immunity is similar in BM and G-PB graft recipients. (A) Serial measurements of T-cell, B-cell, and DC subsets in blood samples from patients stratified by whether they had a previous diagnosis of grade 2-4 aGVHD (orange lines) or grade 0-1 aGVHD (blue lines) (upper panels). Reconstitution kinetics for mean values of B cells (far left and near left lower panels) and pDCs (far right and near right lower panels) stratified by a prior diagnosis of grade 2-4 aGVHD among BM or G-PB transplant recipients. (B) Intracellular expression of IL-2, IFN-γ, and TNF-α in CD4⁺ and CD8⁺ T cells from recipients of BM and G-PB grafts. Percentages of CD4⁺ and CD8⁺ T cells that synthesized IL-2 in response to antigenic nonspecific activation with PMA/ionomycin (top row). Percentages of CD4⁺ and CD8⁺ T cells that expressed IL-2 (second row), IFN-γ (third row), or TNF-α (bottom row) following overnight coculture with CMV lysate. pts, patients.