Deletion of platelet CypD reduces infarct size and improves neurologic and motor functions and blood flow following cerebral ischemia-reperfusion injury. Male and female platelet-specific CypD-deficient (CypD^plt−/−) mice or littermate controls (CypD^plt+/+) were subjected to transient (eg, 1 hour) cerebral ischemia, followed by 23 hours of reperfusion via tMCAO. (A) Representative brain sections stained with 2,3,5-triphenyl tetrazolium chloride. Healthy tissue stains red, whereas an absence of staining (white; arrows) indicates infarcted areas. (B) Brain infarct volumes were quantified by planimetric analysis. (C) Neurological outcome was assessed using the Bederson test; a lower score indicates better functioning. (D) Motor function was examined using the grip strength test; a higher score indicates better functioning. Brain infarct volumes in male (E) and female (F) mice 24 hours after tMCAO. (G) CBF was monitored longitudinally in the right MCA territory (the same side as occlusion) before ischemia (baseline), during ischemia, at the start of reperfusion, and at 3 and 24 hours after stroke onset.