MDMs treated with IL-10 show increased CD163 expression and increased ADAMTS13 endocytosis. (A) MMR and (B) CD163 expression by DCs (dotted line), MDM (MΦ) (dashed line), and IL-10–treated MDM (MΦ + IL-10) (solid red line). Blue area represents isotype control. (C) ADAMTS13-AF488 uptake by IL-10–treated MDMs. MDMs incubated without ADAMTS13-AF488 (blue area), ADAMTS13-AF488 uptake by DCs (dotted line; red bar), MDMs (dashed line; blue bar), or IL-10-differentiated MDMs (solid red line; green bar) are shown. (D) Blocking of ADAMTS13 uptake by DCs, MDMs (MΦ), and IL-10–treated MDMs (MΦ + IL-10) by EDHu-1, clone 15.2, and a mixture of EDHu-1 and clone 15.2. Data are expressed as percentage of MFI; 100% corresponds to the mean fluorescence signal observed for control-treated MDMs in the absence of any inhibitor. *P < .05; **P < .01; ***P < .001 (Student t test).