Cytogenetic evaluation and next-generation sequencing at baseline and time of transformation to AML. For each section of the figure, the upper graph represents VAF of detectable mutations at the time of diagnosis of MDS and at transformation. Lower graphs represent gain (blue) or loss (red) of chromosomal material and translocations (thin black lines connecting chromosomes) present at diagnosis or transformation. A partial semicircle present in the upper right corner of sexual chromosomes in some patients represents marker chromosomes. Inversions or derivatives are shown in green.