American Society of Hematology

Figure 2.

$Figure 2. Growth-inhibitory effects of Bcl10 overexpression in WEHI-231 cells. WEHI-231 cells overexpressing Bcl10 rapidly lost representation in infected cultures consisting of infectants and noninfectants. Cells were infected with retroviruses coexpressing Bcl10 or its N-terminal CARD (Bcl10-CARD) and GFP via a bicistronic mRNA containing an IRES, under the transcriptional control of the CMV enhancer. The fraction of GFP+ cells in each culture was determined in triplicate by flow cytometry over a period of 10 days. For comparison purposes, the GFP+ fractions were all normalized to the fraction on day 5, when the fraction of GFP+ cells was maximal or near-maximal in each culture. Actual percentage of GFP+ cells at day 5 varied among different cultures from 3% to 25% as a result of nonidentical virus titers during infection. Diamonds indicate vector only; squares, full-length Bcl10; and triangles, Bcl10-CARD domain only.$

Growth-inhibitory effects of Bcl10 overexpression in WEHI-231 cells. WEHI-231 cells overexpressing Bcl10 rapidly lost representation in infected cultures consisting of infectants and noninfectants. Cells were infected with retroviruses coexpressing Bcl10 or its N-terminal CARD (Bcl10-CARD) and GFP via a bicistronic mRNA containing an IRES, under the transcriptional control of the CMV enhancer. The fraction of GFP⁺ cells in each culture was determined in triplicate by flow cytometry over a period of 10 days. For comparison purposes, the GFP⁺ fractions were all normalized to the fraction on day 5, when the fraction of GFP⁺ cells was maximal or near-maximal in each culture. Actual percentage of GFP⁺ cells at day 5 varied among different cultures from 3% to 25% as a result of nonidentical virus titers during infection. Diamonds indicate vector only; squares, full-length Bcl10; and triangles, Bcl10-CARD domain only.

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