Ectopic expression of Notch1-IC affects the ability of EBF to activate target promoters. The luciferase data were attained by transiently transfecting HeLa cells with 40 ng of respective indicated reporter construct and either 200 ng EBF alone or EBF in combination with 40 or 120 ng Notch1-IC-encoding expression plasmid. The data are based on a single representative of 3 experiments including 3 transfections normalized to the internal control pRL-0. The reporter activities obtained with empty expression plasmid were set to 1 and error bars indicate SD.