Notch signaling reduces the DNA-binding activity of EBF in primary CD19⁺ bone marrow cells. (A-B) The resulting autoradiogram of an EMSA experiment where either Oct1 protein-binding site or Cd79a promoter EBF-binding site was incubated with nuclear extracts from CD19⁺ bone marrow cultured on either Fc-TRAIL-R4- or Fc-Delta4-coated plates for 3 or 20 hours as indicated. (C-D) Diagrams display Q-PCR analysis from the CD19⁺ cells used in the EMSA. The relative mRNA expression levels of EBF and Pax-5 are shown. The data are based on 6 PCR experiments and 2 separate stimulations. Error bars indicate SD.