Increased surface expression of CXCR5 and reduced migration toward CCL21 on T cells following activation. (A) Expression of CXCR5 on naive DO11.10 cells and adoptively transferred DO11.10 cells isolated from the dLNs of Balb/c recipients at day 4 after immunization with OVA/CT. CXCR5 expression on KJ1-26⁺ cells is shown. The shaded area represents the isotype staining. Representative data are shown from 2 to 4 mice analyzed at each time point. Similar results were obtained in 1 to 2 additional experiments. (B) DO11.10 cells isolated from the dLNs of Balb/c recipients at day 4 after adoptive transfer and immunization with OVA/CT (▪) or from the spleen of naive DO11.10 donors (□) were used in transwell assay to assess their responsiveness toward different concentrations of CCL21. (Mean + SD; n = 3 independent experiments).