Figure 2.
The effect of PU.1 deletion on IL-7Rα and EBF expression in pre-BII cells. (A) IL-7Rα expression on pre-BII cells. Pre-BII cells (B220+c-Kit-CD25+) from the bone marrow of PU.1Δ/ΔCD19 and PU.1+/+CD19 were analyzed for the expression of IL-7Rα by FACS. (B) Gene expression in pre-BII cells. Pre-BII cells were sorted by FACS, total RNA extracted, and gene expression analyzed by RT-PCR.