Figure 1.
Effect of TPT on the proliferative response of bidirectional MLR and anti-CD3/CD28–stimulated CD4+T cells. Allogeneic human PBMCs from 2 different donors (3 × 105 cells/well each) were cocultured in 96-well flat-bottom plates in the presence of the indicated concentration of TPT. After 5 days, proliferation was measured. Purified CD4+ T cells (105 cells/well) were cultured in the presence of indicated concentration of TPT and stimulated with CD3/CD28 T-cell expansion Dynabeads (5 × 103/well). After 72 hours, proliferation was measured. The data shown are the percent of control group proliferation (without TPT), which are from a representative experiment out of 3 performed. Error bars indicate standard deviation.
