![Figure 4. Inhibition of TPT on the allostimulatory activity of DCs and LPS-stimulated DCs. (A) Human monocytes were cultured with 50 ng/mL GM-CSF and 50 ng/mL IL-4 in the presence of TPT (TPT[D2-7], 2.5 nM) or medium alone (control). After 7 days, DCs were extensively washed, irradiated (30 Gy [3000 rad]), and added at different concentrations to 1 × 105/well purified allogeneic responder T cells in a 96-well flat-bottom plates. (B) In a second type of experiment, TPT2-treated DCs (TPT[D2-7], 2.5 nM) and control DCs were washed and cultured for another 48 hours with LPS (10 ng/mL). Aliquots of control DCs were cultured with LPS in the presence of TPT (TPT[D8-9], 2.5 nM). After 48 hours, DCs were thoroughly washed and irradiated (30 Gy [3000 rad]), and added in graded doses to 1 × 105/well purified allogeneic responder T cells in a 96-well flat-bottom plates. Each group was tested in triplicate, and thymidine incorporation was measured on day 5 by a 15-hour pulse with [3H] thymidine. The data shown are representative of 3 independent experiments with similar results. Error bars indicate SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/7/10.1182_blood-2005-03-0854/6/zh80190584800004.jpeg?Expires=1735531035&Signature=YgQO7G6lJP8mke9~dszdc6Y5if7dZUTzCyljUAnQCoYDIAtmB6hRbkgd4CTP6Seoir9Pzm~DJ0sSiXhh538bYrdOsSgZn0hjYc9O6a0q1s4H0rKGLHsgK4~54td1KnF-iME4cYrFgYno-Z12dEynpcjaKIG3ctIJ5g1SrvqfFNRQlv4OQUWp-gcylss05hKjEHbT-s5En3x8JFwQBDkmXFiRx1-8gIRlU6iO0VXd6tV4DWf4AOLtMQBgqR2b-bpCuyav8~GKXG7X80vnYvgzUUjahhBwTTHDxJa1u3lsUSsIqnCWdiDpDKDW06DP2iIal7TPAuZuKhTUCl0dklcZUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of TPT on the allostimulatory activity of DCs and LPS-stimulated DCs. (A) Human monocytes were cultured with 50 ng/mL GM-CSF and 50 ng/mL IL-4 in the presence of TPT (TPT[D2-7], 2.5 nM) or medium alone (control). After 7 days, DCs were extensively washed, irradiated (30 Gy [3000 rad]), and added at different concentrations to 1 × 105/well purified allogeneic responder T cells in a 96-well flat-bottom plates. (B) In a second type of experiment, TPT2-treated DCs (TPT[D2-7], 2.5 nM) and control DCs were washed and cultured for another 48 hours with LPS (10 ng/mL). Aliquots of control DCs were cultured with LPS in the presence of TPT (TPT[D8-9], 2.5 nM). After 48 hours, DCs were thoroughly washed and irradiated (30 Gy [3000 rad]), and added in graded doses to 1 × 105/well purified allogeneic responder T cells in a 96-well flat-bottom plates. Each group was tested in triplicate, and thymidine incorporation was measured on day 5 by a 15-hour pulse with [3H] thymidine. The data shown are representative of 3 independent experiments with similar results. Error bars indicate SD.
