American Society of Hematology

Figure 2.

$Figure 2. Venus YFP expression during ES cell differentiation on OP9 stroma. (A) RT-PCR analysis of endogenous Runx1 mRNA transcribed from proximal promoter P2, and corresponding Venus expression in undifferentiated ES cells and in Flk-1+ fraction of differentiated ES cells. Above the open elongated triangles are the numbers of PCR cycles; days of ES cell differentiation on OP9 cell layer are indicated. RPL13 indicates ribosomal protein 13 large subunit. (B) Venus expression in ES cell–derived myeloid hematopoietic cells. (C) Venus expression in ES cell–derived B cells.$

Venus YFP expression during ES cell differentiation on OP9 stroma. (A) RT-PCR analysis of endogenous Runx1 mRNA transcribed from proximal promoter P2, and corresponding Venus expression in undifferentiated ES cells and in Flk-1⁺ fraction of differentiated ES cells. Above the open elongated triangles are the numbers of PCR cycles; days of ES cell differentiation on OP9 cell layer are indicated. RPL13 indicates ribosomal protein 13 large subunit. (B) Venus expression in ES cell–derived myeloid hematopoietic cells. (C) Venus expression in ES cell–derived B cells.

This Feature Is Available To Subscribers Only