Phenotypic differences between IL-7Rα⁺and IL-7Rα^-cells. (A) Dot plots gated on total CD8⁺ T cells in which IL-7Rα is plotted against known markers for T-cell subset differentiation, CD28, CD62L, CD57, and CD45RA. Numbers indicate percentages within CD8⁺ T cells in the corresponding quadrants. (B) Overlay histograms of IL-7Rα- cells (shaded histogram) and IL-7Rα⁺ cells (black line) within total CD8⁺ T cells (left) or CMV-specific CD8⁺ T cells (right). Numbers indicate mean fluorescence intensity of perforin (top panels) and Bcl-2 (bottom panels). Representative results of flow cytometric analysis are shown from 4 independent measurements.