Effect of enforced TAL1 expression on the CD34⁺ cells engrafted into the BM of NOD-SCID mice. (A-B) Measurement of the percentage of CD34⁺/CD14^-CD15^-CD19^- (Lin^-) cells by FACS. CD34⁺ cells were first gated for their lack of CD14 and CD15 expression. Proportion of CD34⁺/Lin^- was obtained by measuring the percentage of CD34⁺/CD19^- in the CD34⁺/CD14^-CD15^- population. Shown are the results of 2 representative mice (A) and the summary of the percentage of CD34⁺/Lin^- cells in the nucleated cells of the BM of engrafted individual NOD-SCID mice (B). Median values are indicated in panel B. (C) Gene expression analysis in CD34⁺/Lin^- cells. RT-PCR were performed on RNA extracted from sorted CD34⁺/Lin^- cells using primers that amplify only human cDNA. Results shown are representative of 2 independent experiments. Each measurement was done twice to ensure reproducibility. Numbers in FACS quadrants indicate the percentage of positive cells in a mononucleated cell gate (panel A).