Adhesion and transmigration profiles in WKY rats actively immunized with hMPO or HSA in response to topical CXCL1. Intravital microscopy was performed on rats 6 to 7 weeks after immunization with hMPO (EAV rats) or HSA (control rats). After recording baseline responses of adhesion (A) and transmigration (B), topical CXCL1 (3 × 10^-9 M) or Tyrode balanced salt solution was superfused over the mesentery, and responses were quantified for a further 90 minutes, during which time superfusion of the topical agent was maintained. The data represent mean ± SEM, n = 11 to 13 separate rats per group for CXCL1 and n = 6 to 7 per group for Tyrode solution. Statistically significant differences between different groups of rats are shown by asterisks, ^*P < .05 and ^***P < .001.