HPLC profile of proteoglycans. Metabolically radiolabeled proteoglycans from normal and Hurler MAPCs were subjected to DEAE-Sephacel anion-exchange HPLC. The column was eluted at 1 mL/minute using an increasing NaCl gradient, and 1-mL fractions were collected. The ³⁵S and ³H radioactivities were measured and plotted for each fraction. The proportion of radioactivity in each peak was calculated as a percentage of the total radioactivity in the proteoglycan-containing peaks. DPM indicates disintegrations per minute.