Figure 2.
![Figure 2. Size distribution of HS. HSs were purified separately from each proteoglycan peak that was resolved by anion-exchange HPLC, and subjected to gel filtration chromatography on a CL-6B column, as described in “Materials and methods.” Three regions containing large-, intermediate-, and small-sized molecules were identified, based on the elution profile of normal intracellular HPLC peak no. 1 HS (Kav 0.1-0.4, 0.4-0.55, and 0.55-0.9, respectively [Kav = (elution volume - void volume)/(total volume - void volume)]). The proportion of HS present in each of these regions was calculated as a percentage of the total, for HS from intracellular peak no. 2 as well as HS from HPLC peaks no. 1 and no. 2 from the ECM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/6/10.1182_blood-2005-02-0657/6/zh80180584120002.jpeg?Expires=1735558214&Signature=AjkPj6n2-l7iOgp73n3ZBXtujpbKPfCK9j6qjReRNYj1vE2WPNCu2dpDg-pFVEABt9oR9DBsnVEexae3tNxEBanVlTKpp95~KqJmBiJS4BRbNCoauPXWYKo9nJwjdPou57-zpL-ItgeO-cd-W5z9YeS-CKMCXDhMhjZGNhwE9VlhSXuBfr-eT1ZfGB70gwy8tng-n3FyqiaAvNNcOFpyav8EVeeN-WTD1ua06W0pY5U-susrA7DgLUwEqMpJkkkXPgsJ-eBLdn530PtqDj-vYlj2MTD-c~Jaq9hYvZKdFXng2M4ypgaWRStpYd9i964szoVfryQ5dgeV~09c3n34Ig__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Size distribution of HS. HSs were purified separately from each proteoglycan peak that was resolved by anion-exchange HPLC, and subjected to gel filtration chromatography on a CL-6B column, as described in “Materials and methods.” Three regions containing large-, intermediate-, and small-sized molecules were identified, based on the elution profile of normal intracellular HPLC peak no. 1 HS (Kav 0.1-0.4, 0.4-0.55, and 0.55-0.9, respectively [Kav = (elution volume - void volume)/(total volume - void volume)]). The proportion of HS present in each of these regions was calculated as a percentage of the total, for HS from intracellular peak no. 2 as well as HS from HPLC peaks no. 1 and no. 2 from the ECM.
