Figure 1.
![Figure 1. αDGK activity in NPM/ALK-positive cells. Cell lines were cultured for 30 minutes to 1 hour at 37°C in the absence (-) or presence (+) of either 10 μM R59949 or 1 μM PP2 and lysed thereafter. Total cell lysates (A) or anti-αDGK immunocomplexes (B, upper panel) were analyzed for in vitro DGK enzymatic activity in the presence of [γ-32P] ATP and diolein as exogen substrate; lipid phase was separated by TLC. An aliquot from each anti-αDGK immunoprecipitate was analyzed by SDS-PAGE to ensure that equal amounts of αDGK were present during in vitro kinase assay (B, bottom panel). PA indicates phosphatidic acid.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/6/10.1182_blood-2005-01-0316/6/zh80180583880001.jpeg?Expires=1743019772&Signature=nFeSS2Nj7KhVg7C1bAktdF1SqWhXke9CTFOV8MRE20MR~Sa1OYL3~ocypJCQeApdRLn3dgqmMFRYB29sn6LDAZQ8dMrBAT2eClJ84yBeJIMwBsrEkWhk-zM9Vzm48qFJcZD0HL4GDGT24mTkp5s7XW1Ir6WR0X9M8yZFGeLYVohyRD9PWprQb43fZvukZv3O1aUgBzwUuSHFRbPF42riWShQuZC0pGH8Ffu9d6ZeVpWzlnp~lUcDvHnzOUqS33v9PsTjEa7WyybNsg-r4MIEaTkxL6Ol6A3x2CTfgF4g7Rvu773zWfa8NO8awpC0P~Jq-YJJfZEdhgEF6r-C0MkWXw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
αDGK activity in NPM/ALK-positive cells. Cell lines were cultured for 30 minutes to 1 hour at 37°C in the absence (-) or presence (+) of either 10 μM R59949 or 1 μM PP2 and lysed thereafter. Total cell lysates (A) or anti-αDGK immunocomplexes (B, upper panel) were analyzed for in vitro DGK enzymatic activity in the presence of [γ-32P] ATP and diolein as exogen substrate; lipid phase was separated by TLC. An aliquot from each anti-αDGK immunoprecipitate was analyzed by SDS-PAGE to ensure that equal amounts of αDGK were present during in vitro kinase assay (B, bottom panel). PA indicates phosphatidic acid.
