Figure 6.
![Figure 6. Colocalization of PGRP-S with lysozyme in the NETs. Neutrophils were activated with 100 nM PMA for 30 minutes and stained for DNA (A), PGRP-S (B), and lysozyme (C). For DNA detection, Hoechst 33342 (blue) was used. PGRP-S was detected with anti-human PGRP-S monoclonal antibody (mouse immunoglobulin G [IgG]) and Cy2-conjugated donkey anti-mouse IgG (green). Lysozyme was detected with anti-human lysozyme polyclonal antibody (sheep IgG) and Cy5-conjugated donkey anti-sheep IgG (red). The merged image (D) clearly shows colocalization of PGRP-S with lysozyme in the NETs.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/7/10.1182_blood-2005-02-0530/6/zh80190584830006.jpeg?Expires=1735829381&Signature=J4f5OGt-HtmQRePbRqFk1Eh9h3NNXh23vG4lNy~AZpiyR6X~WBUzHceKN6ro3bXVHYpghrOc04Vq0xwRPJ-c5JwSyzzzPpG0mcpb2O5-1A0YeQGumgs1vb1A6WQ4GyfO3Nyjr-42H5AN8DRT4EFxdIIf~xDsamTsMZsTCwFrZS0dCsz9S5B3j9mfYMexW4Ba2GdfsdYXUL6Uy9iONTaHItPbLOnDQFuA1RG2f6IH39WRqV9wV8qGJKnF0Li-~y4I-Bm1VAuD13txMnTmVmUmRxE5KGYW0l87tVLupyJ4ta0wPT507ePjMZvedSkx9zDcK5tZlz0DtRNL-ZxcGQ1GPQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Colocalization of PGRP-S with lysozyme in the NETs. Neutrophils were activated with 100 nM PMA for 30 minutes and stained for DNA (A), PGRP-S (B), and lysozyme (C). For DNA detection, Hoechst 33342 (blue) was used. PGRP-S was detected with anti-human PGRP-S monoclonal antibody (mouse immunoglobulin G [IgG]) and Cy2-conjugated donkey anti-mouse IgG (green). Lysozyme was detected with anti-human lysozyme polyclonal antibody (sheep IgG) and Cy5-conjugated donkey anti-sheep IgG (red). The merged image (D) clearly shows colocalization of PGRP-S with lysozyme in the NETs.
