Semiquantitative RT-PCR showing a representative change mRNA levels of caspase-8 and Fas in the liver relative to GAPDH after siRNA treatment. Total RNA was isolated from mice 24 hours, 5 days (d5), or 10 days (d10) after CLP or sham surgery. Animals that underwent CLP received normal saline, GFP siRNA, Fas siRNA, or caspase-8 siRNA 30 minutes after surgery. (A) Fas mRNA levels after CLP/Fas siRNA treatment. (B) caspase-8 mRNA levels after CLP/caspase siRNA treatment. Levels are decreased to sham levels at 24 hours, 5 days, and 10 days after CLP/Fas or caspase-8 siRNA treatment. (C) Densitometry analysis of the intensity of mRNA bands for Fas and caspase-8 relative to GAPDH. Experiment was repeated three times.