Protein levels in both the liver and spleen after Fas or caspase-8 treatment as shown by Western blot. Total protein was isolated from mice 24 hours, 5 days (d5), or 10 days (d10) after CLP or sham surgery. Western blot band density (in arbitrary densitometric units, AU) for Fas (A, B) and caspase-8 (C, D). Blots in panels A and C are representative of 3 separate repeat experiments. ▪ represents the liver; □, the spleen. ^*P < .05 versus CLP/saline and CLP/GFP siRNA controls, t test (n = 4/group). Data are presented as mean ± SE of the mean.