American Society of Hematology

Figure 1.

$Figure 1. Analysis of Hfe gene expression. Hfe expression was analyzed in various organs and in isolated cell fractions from spleen and liver. (A) mRNA was isolated from various organs, as indicated, and was used to synthesize cDNA. Hfe mRNA expression was quantified by qRT-PCR and normalized to Actb (graph). The Hfe/Actb × 103 ratio is shown. Conventional RT-PCR products were visualized by ethidium bromide staining in 1.0% agarose gels. (B) Hfe mRNA expression in positively selected splenic macrophages (Mo), B lymphocytes (B), T lymphocytes (T), NK cells, and (C) whole liver (liver), liver macrophages (Mo), and mononuclear cells (MNC) assessed by qRT-PCR and normalized to Actb. The Hfe/Actb × 103 ratio is shown. Results are presented as mean ± SD (n = 3 mice per organ or cell isolate).$

Analysis of Hfe gene expression.Hfe expression was analyzed in various organs and in isolated cell fractions from spleen and liver. (A) mRNA was isolated from various organs, as indicated, and was used to synthesize cDNA. Hfe mRNA expression was quantified by qRT-PCR and normalized to Actb (graph). The Hfe/Actb × 10³ ratio is shown. Conventional RT-PCR products were visualized by ethidium bromide staining in 1.0% agarose gels. (B) Hfe mRNA expression in positively selected splenic macrophages (Mo), B lymphocytes (B), T lymphocytes (T), NK cells, and (C) whole liver (liver), liver macrophages (Mo), and mononuclear cells (MNC) assessed by qRT-PCR and normalized to Actb. The Hfe/Actb × 10³ ratio is shown. Results are presented as mean ± SD (n = 3 mice per organ or cell isolate).

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