Hypoferremia is induced by hHep-25 but not its truncated variants. Mice received a single 50 μg injection of hHep-25, diluent, hHep-22, or hHep-20. Only the full-length, 25-amino acid hHep induced hypoferremia at 2 hours (n = 4 mice for every treatment). Means and standard deviations are shown, and comparisons indicated by solid lines, and P values were tested by Student t test.